Anti-bovine IFN-γ mAb (MT307), PF647P
Anti-bovine IFN-γ mAb (MT307), PF647P
Intended use
This monoclonal antibody enables specific detection of bovine IFN-γ-producing cells in flow cytometry.
Serum/Plasma samples
Recommendation
5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.
Product details
| Product | Anti-bovine IFN-γ mAb (MT307), PF647P |
| Application | Flow cytometry |
| Analyte | IFN-γ |
| Antibody | MT307 |
| Conjugate | PF647P |
| Clonality | Monoclonal |
| Immunogen | Recombinant bovine IFN-γ |
| Host | Mouse |
| Isotype | IgG2a |
| Reactivity | Cow, Sheep, Goat |
| Specificity | Native and recombinant bovine (cow) IFN-γ |
| Cross-reactivity | Cross-reacts with IFN-γ from sheep and goat. For more information, please check the Veterinary cross-reactivity guide. |
| Supplied in | PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm). |
| Contents | Monoclonal antibody MT307, conjugated to PF647P fluorophore. Ready to use. |
Shipping and Storage
| Shipping | Shipped at ambient temperature. |
| Storage | Store product at 4-8 °C. |
| Shelf life | At least 12 months from date of receipt. |
Detection of IFN-γ by flow cytometry in viable bovine PBMC. Stimulated, fixed and permeabilized cells were stained with anti-bovine mAb MT307-PF647P (left panel). MT307-PF647P blocked with recombinant antigen prior to labelling of cells (middle panel). Matched isotype control antibody (right panel). Flow cytometry was performed on a BD FACSVerse system.
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