Catalog / ELISA kits / TNF-α ELISA kits

TNF-α ELISA kits

We make ELISA kits for TNF-α in 5 species. This page explains how they differ, so you can pick the one your experiment needs.

What differs between these kits

Which species they are for

Available for Bovine, Human, Monkey, Mouse, and Porcine. The species decides which antibody pair we supply, so it is the first thing to choose. Some kits are also validated on closely related species, so if you work in one we do not list here, check the reactivity on the product page.

Whether a pre-coated plate is included

Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

About TNF-α

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Also known asTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF
Produced byT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
SpeciesBovine, Human, Monkey, Mouse, Porcine
Gene IDs
Bos taurus: 280943
Homo sapiens: 7124
Mus musculus: 21926

Find the right TNF-α kit

Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.

Species

What you are measuring in

Pre-coated plate included

Or coat your own

Kit detection system

Which enzyme conjugate we supply


Start by choosing a species.


Frequently bought together

What customers usually order alongside these kits, whichever species they work in.

ELISA substrate: pNPP for ALP

Substrates

ELISA substrate: pNPP for ALP


Related reading

Guides and tutorials covering TNF-α and the ELISA method.

Tutorials

Tutorial

2025-04-08

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