ELISA Pro: Mouse IL-12/-23 p40
ELISA Pro: Mouse IL-12/-23 p40
Components
| Detection mAb | C17.8, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Standard | Recombinant mouse IL-12 p70 ELISA standard |
| Substrate | ELISA substrate: TMB for HRP |
| Plate | Pre-coated ELISA strip plate (mAb C15.6) |
| Also provided | Adhesive plate covers |
| Buffers/Solutions | ELISA diluent |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution |
In stock
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Performance
| Standard range | 10-3160 pg/ml |
| Sensitivity | 4 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 8 standard deviations to the mean OD of background wells. |
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Analyte information
IL-12/-23 (p40)
| Analyte description | Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells. Interleukin-23 (IL-23) is a disulfide-linked heterodimer that consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells, and IL-23 is responsible for Th17 expansion and stabilization. Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers. |
| Alternative names | Interleukin 12, IL-12, IL12, IL12A, IL12B, Il-12b, Il-12p40, Il12p40, p40 |
| Cell type | Monocyte/MΦ, mDC |
| Gene ID | 16160 |