Isotype control mAb (MT128) for mouse IgG1, PF488P

3612-71-100T

Publications: 0

Documents

Questions?

Validated for our assays.

Content

Monoclonal antibody MT128, conjugated to PF488P fluorophore. Ready to use.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This monoclonal antibody is recommended as mouse IgG1 isotype control in flow cytometry. It has been used as isotype control with cells from different species.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm.

Product details

Product	Isotype control mAb (MT128) for mouse IgG1, PF488P
Application	Flow cytometry, Isotype control
Analyte	Non-specific
Antibody	MT128
Conjugate	PF488P
Clonality	Monoclonal
Immunogen	Bacterial antigen
Host	Mouse
Isotype	IgG1
Specificity	Bacterial protein
Supplied in	PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT128, conjugated to PF488P fluorophore. Ready to use.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Profile of human lymphocytes stained with mouse IgG1 isotype control antibody MT128 (dashed line) and anti-human perforin antibody (solid line). Cells were fixed and permeabilized prior to staining. The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Documents

Datasheet/Protocol
SDS

Publications (0)

Loading publications...

Analyte information

Non-specific

Analyte description

Isotype controls are often used as negative controls in flow cytometry, immunochemistry, or Western blot experiments. An isotype control is an antibody that doesn't bind the target analyte but otherwise matches the properties of the primary antibody. For example, an isotype control should be conjugated in the same way and match the isotype of the target-specific antibody used in the assay.