ELISA Pro: Human Thioredoxin‑1
ELISA Pro: Human Thioredoxin‑1
Components
| Plate | Pre-coated ELISA strip plate (mAb MT17R6) |
| Detection mAb | MT13X3, biotin |
| Enzyme conjugate | Streptavidin-HRP enzyme conjugate |
| Standard | Recombinant human thioredoxin-1 ELISA standard |
| Substrate | TMB substrate for ELISA |
| Buffers/Solutions | Assay buffer |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution | |
| Also provided | Adhesive plate covers |
Product specifications
Intended use
This ELISA Pro kit is intended for the quantification of reduced and oxidized forms of human Thioredoxin-1 (Trx1) in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
The Assay buffer included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The Assay buffer has been validated using serum/plasma samples from healthy human blood donors.
Recommendation
Product details
| Product | ELISA Pro: Human Thioredoxin-1 |
| Application | ELISA |
| Analyte | Thioredoxin-1 |
| Reactivity | Human |
| Specificity | This kit is based on a matched pair of mAbs specific for human Thioredoxin-1 (Trx1). Reduced and oxidized forms of Trx1 are detected. |
Shipping and Storage
| Shipping | Shipped at ambient temperature. |
| Storage | Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature. |
| Shelf life | At least 12 months from date of receipt. |
Performance
| Standard range | 31.2-2000 pg/ml |
| Sensitivity | 15 pg/ml The limit of detection is the lowest concentration that is possible to detect but not necessarily quantify with precision and accuracy. |
Tutorials
