ELISA Pro: Human Thioredoxin-1

ELISA Pro: Human Thioredoxin-1

3580-1HP-1

Publications: 5
Documents
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAbMT13X3, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human thioredoxin-1 ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb MT17R6)
Also providedAdhesive plate covers
Buffers/SolutionsAssay buffer
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

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Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of reduced and oxidized forms of human Thioredoxin-1 (Trx1) in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using from healthy human blood donors.

Recommendation

Product details

ProductELISA Pro: Human Thioredoxin-1
ApplicationELISA
AnalyteThioredoxin-1
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human Thioredoxin-1 (Trx1). Reduced and oxidized forms of Trx1 are detected.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range31.2-2000 pg/ml
Sensitivity15 pg/ml

The limit of detection is the lowest concentration that is possible to detect but not necessarily quantify with precision and accuracy.

Representative standard curve for human Thioredoxin-1 (Trx1) ELISA Pro kit using TMB substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.
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Thioredoxin-1

Analyte description

Thioredoxin-1 (Trx1) is an oxidoreductase protein and thus may act as an anti-oxidant by facilitating the reduction of cysteine residues of other proteins. Besides keeping the intracellular milieu in a reduced state and being involved in the defence against oxidative stress, thioredoxin-1 specifically regulates several transcription factors and receptors. Increased plasma levels of thioredoxin-1 has been associated with several psychiatric disorders.

Alternative namesThioredoxin-1, Trx1, TRDX, TRX, TRX1, Trx80
Cell typeT cell, Tc, Th1, Th2, Th17, Treg, Monocyte/MΦ
Gene ID7295