ELISA Flex: Human IL-8 (ALP)

ELISA Flex: Human IL-8 (ALP)

3560-1A-6

Publications: 25
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT8H6
Detection mAbMT8F19, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human IL-8 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-8 (CXCL8) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

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Product details

ProductELISA Flex: Human IL-8 (ALP)
ApplicationELISA
AnalyteIL-8 (CXCL8)
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-8 (CXCL8).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range4-400 pg/ml
Representative standard curve for human IL-8 (CXCL8) ELISA Flex kit using pNPP substrate.
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IL-8 (CXCL8)

Analyte description

Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.

Alternative namesInterleukin-8, IL-8, IL8, CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8
Cell typeT cell, Treg, Tfh, Monocyte/MΦ, mDC
Gene ID3576
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