ELISA Flex: Human IL‑1α (ALP)

Standard curve for human IL-1α ELISA Flex kit.

ELISA Flex: Human IL‑1α (ALP)

3414-1A-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT641/642
Detection mAb	MT513, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Standard	Recombinant human IL-1α ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

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Frequently bought together

1 / 2

ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human IL-1α (pro-form and active form) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IL-1α (ALP)
Application	ELISA
Analyte	IL-1α
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-1α (pro-form and active form).
Cross-reactivity	The assay shows no cross-reactivity with recombinant human IL-1β or IL-18.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

2-200 pg/ml

Representative standard curve for human IL-1α ELISA Flex kit using pNPP substrate.

Datasheet/Protocol SDS

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IL-1α

Analyte description	Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation.
Alternative names	Interleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1
Cell type	Monocyte/MΦ, mDC
Gene ID	3552