ELISA Pro: Human IL‑10

Standard curve for human IL-10 ELISA Pro kit.

ELISA Pro: Human IL‑10

3430-1HP-1

Publications: 82

Documents

Bulk or custom format

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb 9D7)
Detection mAb	12G8, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IL-10 ELISA standard
Substrate	TMB substrate for ELISA
Buffers/Solutions	ELISA diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This ELISA Pro kit is intended for the quantification of human IL-10 in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.

Recommendation

Product details

Product	ELISA Pro: Human IL-10
Application	ELISA
Analyte	IL-10
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-10.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Standard range	3.16-1000 pg/ml
Sensitivity	1 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of human Interleukin-10 (IL-10) ELISA Pro kit.

Datasheet/Protocol SDS

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IL-10

Analyte description	Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Alternative names	Interleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell type	T cell, Treg, Monocyte/MΦ, mDC
Gene ID	3586

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ELISA PathRF: Human IL-10