ELISA Pro: Human IFN-γ

ELISA Pro: Human IFN-γ

3420-1HP-1

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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IFN-γ ELISA standard
SubstrateELISA substrate: TMB for HRP
PlatePre-coated ELISA strip plate (mAb 1-D1K)
Also providedAdhesive plate covers
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human IFN-γ in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using from healthy human blood donors.

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Product details

ProductELISA Pro: Human IFN-γ
ApplicationELISA
AnalyteIFN-γ
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.
Standard range2-1000 pg/ml
Sensitivity1 pg/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells.
Mean standard curve from repeated runs of human Interferon-gamma (IFN-γ) ELISA Pro kit.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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