ELISA Pro: Human IFN‑γ

Standard curve for human IFN-γ ELISA Pro kit.

Model of human IFN-γ protein showing the epitopes for monoclonal antibodies 1-D1K (blue) and 7-B6-1 (orange).

ELISA Pro: Human IFN‑γ

3420-1HP-1

Publications: 259

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Bulk or custom format

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb 1-D1K)
Detection mAb	7-B6-1, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IFN-γ ELISA standard
Substrate	TMB substrate for ELISA
Buffers/Solutions	ELISA diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This ELISA Pro kit is intended for the quantification of human IFN-γ in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.

Recommendation

Product details

Product	ELISA Pro: Human IFN-γ
Application	ELISA
Analyte	IFN-γ
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Standard range	2-1000 pg/ml
Sensitivity	1 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of human Interferon-gamma (IFN-γ) ELISA Pro kit.

Datasheet/Protocol SDS

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IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3458

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ELISA PathRF: Human IFN-γ