Goat anti-human IgG, biotin

3820-4-250

Publications: 94

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Validated for our assays.

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Intended use

This antibody enables specific detection of human IgG in enzyme immunoassays such as indirect ELISA.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

Product	Goat anti-human IgG, biotin
Application	ELISA
Analyte	IgG
Antibody	Goat anti-human IgG
Conjugate	Biotin
Clonality	Polyclonal
Immunogen	Native human IgG
Host	Goat
Reactivity	Human
Specificity	Human IgG H-chains (Fcγ)
Cross-reactivity	No cross-reactivity with human IgA, IgM or non-immunoglobulin serum proteins. May cross-react with immunoglobulins from other species.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Goat anti-human IgG, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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IgG

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Alternative names	Immunoglobulin G, IgG, IgG3
Cell type	B cell
Gene ID	3500, 3501, 3502