Goat anti-human IgG, ALP
Goat anti-human IgG, ALP
Product specifications
Intended use
This ALP-conjugated antibody enables specific detection of human IgG in enzyme immunoassays such as indirect ELISA and Western blot.
Serum/Plasma samples
Recommendation
Dilute 1/1000 as a general guideline. Dilution factor may vary with differing assay conditions.
Product details
| Product | Goat anti-human IgG, ALP |
| Application | ELISA, Western blot |
| Analyte | IgG |
| Antibody | Goat anti-human IgG |
| Conjugate | ALP |
| Clonality | Polyclonal |
| Immunogen | Native human IgG |
| Host | Goat |
| Reactivity | Human |
| Specificity | Human IgG H-chains (Fcγ) |
| Cross-reactivity | No reactivity with human IgA, IgM, or mouse Ig. May cross-react with IgG from other species. |
| Supplied in | 0.1 M Tris buffer with 1% BSA and 0.002% Kathon CG. |
| Contents | Goat anti-human IgG, ALP-conjugated. Supplied in 0.1 M Tris buffer with 1% BSA and 0.002% Kathon CG. |
Shipping and Storage
| Shipping | Shipped at ambient temperature. |
| Storage | Store product at 4-8 °C. |
| Shelf life | At least 18 months from date of receipt. |
Human IgG mAb guide
Learn how to combine Mabtech’s antibodies for human IgG ELISA and ELISpot. Adjust the setup to detect either total IgG or antigen-specific IgG. Different antibody combinations of monoclonal antibodies have been selected for the detection of the human IgG subclasses in ELISA and ELISpot. There is no cross-reactivity to human IgA1, IgA2, IgM, and IgE.
Application guide: ELISA
The human IgG ELISA can be adapted to detect antigen-specific IgG or the total IgG in serum or plasma samples.
Antigen-specific IgG
Capture: Antigen
Detection mAbs: MT78/145, biotin or MT78, ALP
Total IgG
Capture mAb: MT145, unconjugated
Detection mAb: MT78, ALP
Application guide: ELISpot
The human IgG ELISpot and FluoroSpot can be adapted to detect antigen-specific IgG-secreting cells or the total IgG-secreting cells in cell samples like PBMCs.
Antigen-specific IgG
Capture:
Antigen
Detection mAbs:
MT78/145, biotin
Capture mAbs:
MT91/145, unconjugated
Detection:
Antigen-biotin
Total IgG
Capture mAbs:
MT91/145, unconjugated
Detection mAbs:
MT78/145, biotin
Get inspired!
Make sure to check out how researchers have used Mabtech's human IgG ELISA, ELISpot, and FluoroSpot kits in everything from vaccine development to SARS-CoV-2 research. You can find it all in our Publication database!