FociSpot Path: SARS-CoV-2 (N)

FociSpot Path: SARS-CoV-2 (N)

4401-4AT-1

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Content

Detection mAbMTCC25, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Plate96-well tissue culture treated plate

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This kit is intended for FociSpot immunostaining of the SARS-CoV-2 nucleocapsid (N) protein, which enables enumeration of SARS-CoV-2 virus foci in Focus Forming Assays (FFA). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

FociSpot
In FociSpot, virus foci are detected by using mAbs specific to viral proteins. After fixation and permeabilization of infected cells in a 96-well plate, the virus-specific biotinylated detection mAb is added. The use of Streptavidin-ALP enzyme conjugate, followed by a precipitating substrate enables detection of foci. Foci can be counted manually, but counting with Mabtech IRIS 2 is rapid and offers easy data handling.

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Product details

ProductFociSpot Path: SARS-CoV-2 (N)
ApplicationFociSpot
AnalyteSARS-CoV-2 (N)
Specificity

SARS-CoV-2 nucleocleocapsid

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibody, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/Protocol
SDS

Tutorial, Published October 12, 2023

Step-by-step guide to FociSpot
FociSpot is our new assay platform that includes both kits and a brand-new analysis capability of Mabtech IRIS™ 2 allowing for automated counting of foci in a 96-well plate format. Let us show you how you can run an FRNT50 with FociSpot.
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SARS-CoV-2 (N)

Analyte description

The nucleocapsid (N) protein is a structural protein of the SARS-CoV-2 virus. It is responsible for packaging the positive-sense RNA genome by forming ribonucleoprotein (RNP) structures with the viral RNA. The N protein is mainly expressed in the host cells' cytoplasm and interacts with the M protein during assembly. The nucleocapsid protein is highly immunogenic and antibodies against it are found in people that recovered from COVID-19. The N protein has been suggested to suppress type I interferon responses. 

Alternative namesNucleocapsid protein, N protein (of SARS-CoV-2)