EYRAplex Flex: Mouse Cytokine/Chemokine, pre‑mix

Introduction

EYRAplex Flex is a custom magnetic bead-based multiplex assay for the simultaneous detection and quantification of multiple analytes in a single sample. The magnetic beads have been dyed with different concentrations of two fluorophores, resulting in distinct bead populations with unique bead IDs. Each bead population has been conjugated with a specific monoclonal antibody, allowing specific binding to its corresponding analyte. Multiplex analysis is enabled by combining several bead populations that are supplied as a capture bead mix. Please see the included Certificate of Analysis for your kit's panel-specific details. 

In EYRAplex, the capture bead mix is added to wells in a 96-well plate. Samples and standards are then added. During incubation, the capture antibodies bind to analytes present in the sample. Detection occurs in two steps. First, a biotin-labeled monoclonal antibody mix (detection mAb mix) is added. These detection antibodies bind to the captured analytes. Streptavidin-PE is then added, binding to the biotin and generating a fluorescent signal proportional to the analyte concentration. 

The fluorescent signal from each bead ID determines the analyte, while the streptavidin-PE signal reflects its quantity. Analytes are quantified by comparing the signals to standard curves.