EYRAplex Flex: Human Cytokine/Chemokine, pre-mix

EYRAplex Flex: Human Cytokine/Chemokine, pre‑mix

EYX-HCC-PM-1-1

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Components

Capture mAb	Capture bead mix: Human Cytokine/Chemokine (1-plex)
Detection mAb	Detection mAb mix: Human H1A

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Intended use

EYRAplex Flex, pre-mix is intended for the simultaneous quantification of multiple analytes in serum, plasma, and cell culture supernatants using multiplex bead-based immunoassay technology. The specific analytes measured are determined by the selected capture bead configuration. The assay is designed for analysis using Mabtech EYRA™, but can also be used in flow cytometry. For research use only. Not for use in diagnostic procedures.

Introduction

EYRAplex Flex is a custom magnetic bead-based multiplex assay for the simultaneous detection and quantification of multiple analytes in a single sample. The magnetic beads have been dyed with different concentrations of two fluorophores, resulting in distinct bead populations with unique bead IDs. Each bead population has been conjugated with a specific monoclonal antibody, allowing specific binding to its corresponding analyte. Multiplex analysis is enabled by combining several bead populations that are supplied as a capture bead mix. Please see the included Certificate of Analysis for your kit's panel-specific details.

In EYRAplex, the capture bead mix is added to wells in a 96-well plate. Samples and standards are then added. During incubation, the capture antibodies bind to analytes present in the sample. Detection occurs in two steps. First, a biotin-labeled monoclonal antibody mix (detection mAb mix) is added. These detection antibodies bind to the captured analytes. Streptavidin-PE is then added, binding to the biotin and generating a fluorescent signal proportional to the analyte concentration.

The fluorescent signal from each bead ID determines the analyte, while the streptavidin-PE signal reflects its quantity. Analytes are quantified by comparing the signals to standard curves.

Serum/Plasma samples

EYRAplex Flex is designed to minimize interference from heterophilic antibodies and rheumatoid factor (RF) in human samples, which can lead to false-positive results in immunoassays. The assay has been validated using serum and plasma samples from healthy donors, as well as samples containing elevated RF levels.

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