ELISpot Path: TBEV (C+E) Human IFN‑γ
ELISpot Path: TBEV (C+E) Human IFN‑γ
$670
Components
| Plate | Pre-coated ELISpot plate, strip (mAb 1-D1K) |
| Detection mAb | 7-B6-1, biotin |
| Enzyme conjugate | Streptavidin-ALP enzyme conjugate for ELISpot |
| Substrate | BCIP/NBT-plus substrate for ELISpot |
| Positive control | Anti-CD3 mAb (CD3-2) |
| Peptide pool | TBEV (C+E), scanning |
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Performance
Human PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: TBEV (C+E), scanning and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS.
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69 |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 3458 |