ELISA Pro: Human Granzyme B

Standard curve for human Granzyme B ELISA Pro kit.

ELISA Pro: Human Granzyme B

3486-1HP-1

Publications: 77

Documents

Bulk or custom format

Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.

Components

Plate	Pre-coated ELISA strip plate (mAb MT34B6)
Detection mAb	MT28, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human granzyme B ELISA standard
Substrate	TMB substrate for ELISA
Buffers/Solutions	ELISA diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This ELISA Pro kit is intended for the quantification of human granzyme B in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.

Recommendation

Product details

Product	ELISA Pro: Human Granzyme B
Application	ELISA
Analyte	Granzyme B
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Standard range	10-3160 pg/ml
Sensitivity	5 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 4 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of human Granzyme B ELISA Pro kit.

Datasheet/Protocol SDS

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Granzyme B

Analyte description	Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
Alternative names	Granzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3002