ELISA Pro: Bovine IFN-γ
ELISA Pro: Bovine IFN-γ
Publications: 73
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Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.
Components
| Detection mAb | MT307, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Standard | Recombinant bovine IFN-γ ELISA standard |
| Substrate | ELISA substrate: TMB for HRP |
| Plate | Pre-coated ELISA strip plate (mAb MT17.1) |
| Also provided | Adhesive plate covers |
| Buffers/Solutions | ELISA diluent |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution |
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Performance
| Standard range | 3.16-1000 pg/ml |
| Sensitivity | 0.5 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 8 standard deviations to the mean OD of background wells. |
Mean standard curve from repeated runs of bovine IFN-γ ELISA Pro kit.
Documents
Veterinary cross-reactivity guide
Tutorials
Publications (73)
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Analyte information
IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 281237, 443396 |