ELISA Path: SARS‑CoV‑2 (RBD) Total Antibody
ELISA Path: SARS‑CoV‑2 (RBD) Total Antibody
Components
Plate | ELISA strip-plate (uncoated) |
Antigen | RBD-bridge mix |
Enzyme conjugate | Anti-WASP mAb, HRP |
Substrate | TMB substrate for ELISA |
Buffers/Solutions | Strep-Tactin®XT |
Coating buffer | |
Dilution buffer | |
Wash buffer concentrate | |
Stop solution | |
Also provided | Adhesive plate covers |
Product specifications
Intended use
This ELISA Path kit is intended for the quantification of Ig to SARS-CoV-2 RBD in serum and plasma. For research use only. Not for use in diagnostic procedures.
Serum/Plasma samples
ELISA for detection of antibodies to the SARS-CoV-2 Receptor Binding Domain (RBD)
This ELISA assay employs a bridging technology for detection of antibodies (immunoglobulins IgG, IgA, IgM) to the SARS-CoV-2 Receptor Binding Domain (RBD) in solutions, e.g., serum/plasma. The kit contains all reagents and buffers needed for the assay. It also contains one uncoated ELISA strip-plate. The ELISA has been validated with human serum/plasma samples from COVID-19 convalescent individuals and uninfected control individuals. The ELISA is species independent. Analysis of serum/plasma samples from uninfected mouse, cynomolgus macaque, rhesus macaque, rat, cat, cow, dog, rabbit, and pig, all resulted in OD values below cut off.
Assay principle
The ELISA plate is coated (overnight) with Strep-Tactin®XT. Sample and the RBD-bridge mix are added after blocking. The RBD-bridge mix contains two components, recombinant RBD expressed with a Twin-Strep-tag®, and recombinant RBD expressed with WASP peptide tag. Antibodies with specificity to RBD will bind RBD-Twin-Strep-tag® with one arm and RBD-WASP tag with the other, creating a bridge. The Twin-Strep-tag will bind to the coated Strep-Tactin®XT, allowing the whole bridge complex to be captured. The captured complex is detected by addition of anti-WASP-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and optical density determined using an ELISA plate reader.
Recommendation
Product details
Product | ELISA Path: SARS-CoV-2 (RBD) Total Antibody |
Application | ELISA |
Analyte | Ig |
Reactivity | Human |
Specificity | IgG, IgA, and IgM specific to the SARS-CoV-2 Receptor Binding Domain (RBD |
Shipping and Storage
Shipping | Shipped at ambient temperature. |
Storage | All reagents except the RBD-bridge mix should be stored at 4-8 °C upon receipt. The RBD-bridge mix should be stored at -20 °C. Plate may be kept at room temperature. |
Shelf life | At least 12 months from date of receipt. |
Performance
Standard range | ng/ml |
Product highlights