ELISA Path: SARS‑CoV‑2 (RBD) Total Antibody

ELISA Path: SARS‑CoV‑2 (RBD) Total Antibody

Components

PlateELISA strip-plate (uncoated)
AntigenRBD-bridge mix
Enzyme conjugateAnti-WASP mAb, HRP
SubstrateTMB substrate for ELISA
Buffers/SolutionsStrep-Tactin®XT
Coating buffer
Dilution buffer
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

Intended use

This ELISA Path kit is intended for the quantification of Ig to SARS-CoV-2 RBD in serum and plasma. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA for detection of antibodies to the SARS-CoV-2 Receptor Binding Domain (RBD)
This ELISA assay employs a bridging technology for detection of antibodies (immunoglobulins IgG, IgA, IgM) to the SARS-CoV-2 Receptor Binding Domain (RBD) in solutions, e.g., serum/plasma. The kit contains all reagents and buffers needed for the assay. It also contains one uncoated ELISA strip-plate. The ELISA has been validated with human serum/plasma samples from COVID-19 convalescent individuals and uninfected control individuals. The ELISA is species independent. Analysis of serum/plasma samples from uninfected mouse, cynomolgus macaque, rhesus macaque, rat, cat, cow, dog, rabbit, and pig, all resulted in OD values below cut off.

Assay principle
The ELISA plate is coated (overnight) with Strep-Tactin®XT. Sample and the RBD-bridge mix are added after blocking. The RBD-bridge mix contains two components, recombinant RBD expressed with a Twin-Strep-tag®, and recombinant RBD expressed with WASP peptide tag. Antibodies with specificity to RBD will bind RBD-Twin-Strep-tag® with one arm and RBD-WASP tag with the other, creating a bridge. The Twin-Strep-tag will bind to the coated Strep-Tactin®XT, allowing the whole bridge complex to be captured. The captured complex is detected by addition of anti-WASP-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and optical density determined using an ELISA plate reader.

Recommendation

Product details

ProductELISA Path: SARS-CoV-2 (RBD) Total Antibody
ApplicationELISA
AnalyteIg
ReactivityHuman
Specificity

IgG, IgA, and IgM specific to the SARS-CoV-2 Receptor Binding Domain (RBD

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

All reagents except the RBD-bridge mix should be stored at 4-8 °C upon receipt. The RBD-bridge mix should be stored at -20 °C. Plate may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard rangeng/ml

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