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ELISA Flex: Porcine IL‑2 (ALP)

Standard curve for Porcine IL-2 ELISA Flex kit.

ELISA Flex: Porcine IL‑2 (ALP)

3140-1A-6

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Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT264/267
Detection mAbMT265, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
StandardRecombinant porcine IL-2 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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ELISA substrate: pNPP for ALP

Intended use

This ELISA Flex kit is intended for the quantification of porcine IL-2 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Porcine IL-2 (ALP)
ApplicationELISA
AnalyteIL-2
ReactivityPig
SpecificityThis kit is based on a matched pair of mAbs specific for porcine (pig) IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.

Standard range3-1000 pg/ml
Standard curve for Porcine IL-2 ELISA Flex kit.
Representative standard curve for Porcine IL-2 ELISA Flex kit using pNPP substrate.

Datasheet/ProtocolSDS

Tutorial

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.
Tutorial

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, POIL2, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID396868