ELISA Flex: Mouse IgEa (HRP)

ELISA Flex: Mouse IgEa (HRP)

3816-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT18E
Detection mAbMT44E, biotin
Enzyme conjugateStreptavidin-HRP
StandardNative mouse IgE ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IgEa (allotype a) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Mouse IgE allotypes
This kit specifically detects the IgEa allotype. Mouse strains with the IgEa allotype include e.g., AKR, BALB/c, C3H/He, and CBA. Mouse strains with the IgEb allotype include e.g., C57BL/6, C57BL/10, and SJL.

Recommendation

Product details

ProductELISA Flex: Mouse IgEa (HRP)
ApplicationELISA
AnalyteIgEa
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IgEa (allotype IgEa).

Cross-reactivityThe mAbs do not detect mouse IgEb (allotype b).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range30-3000 pg/ml
Representative standard curve for mouse IgEa (IgE allotype a) ELISA Flex kit using TMB substrate.

Tutorial, Published November 28, 2022

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Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IgEa

Analyte description

Immunoglobulin E (IgE) E is secreted by plasma cells as a monomer during the adaptive immune response. IgE antibodies are believed to play an important role in the immune defense against parasitic infections and in hypersensitivity type I reactions upon re-exposure to a specific allergen by binding to and cross-linking Fc receptors (FcεRI) expressed on mast cells and basophils. Allergen-specific IgE is the basis for many in vitro assays for allergy diagnosis. In mice, two allotypes exist: IgEa and IgEb. IgEa differs by 12 single-base changes from IgEb and is present in different mouse strains.

Alternative namesImmunoglobulin Ea, IgEa, Gm900
Cell typeB cell
Gene ID380792