ELISA Flex: Mouse IgEa (ALP)

Standard curve for mouse IgEa (IgE allotype a) ELISA Flex kit.

ELISA Flex: Mouse IgEa (ALP)

3816-1A-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT18E
Detection mAb	MT44E, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Standard	Native mouse IgE ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Frequently bought together

1 / 1

ELISA substrate: pNPP for ALP

Intended use

This ELISA Flex kit is intended for the quantification of mouse IgEa (allotype a) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Mouse IgE allotypes
This kit specifically detects the IgEa allotype. Mouse strains with the IgEa allotype include e.g., AKR, BALB/c, C3H/He, and CBA. Mouse strains with the IgEb allotype include e.g., C57BL/6, C57BL/10, and SJL.

Recommendation

Product details

Product	ELISA Flex: Mouse IgEa (ALP)
Application	ELISA
Analyte	IgEa
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IgEa (allotype IgEa).
Cross-reactivity	The mAbs do not detect mouse IgEb (allotype b).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

30-3000 pg/ml

Representative standard curve for mouse IgEa (IgE allotype a) ELISA Flex kit using pNPP substrate.

Datasheet/Protocol SDS

Tutorial

2023-09-20

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

2022-11-28

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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IgEa

Analyte description	Immunoglobulin E (IgE) E is secreted by plasma cells as a monomer during the adaptive immune response. IgE antibodies are believed to play an important role in the immune defense against parasitic infections and in hypersensitivity type I reactions upon re-exposure to a specific allergen by binding to and cross-linking Fc receptors (FcεRI) expressed on mast cells and basophils. Allergen-specific IgE is the basis for many in vitro assays for allergy diagnosis. In mice, two allotypes exist: IgEa and IgEb. IgEa differs by 12 single-base changes from IgEb and is present in different mouse strains.
Alternative names	Immunoglobulin Ea, IgEa, Gm900
Cell type	B cell
Gene ID	380792