ELISA Flex: Mouse IFN-α (HRP)

ELISA Flex: Mouse IFN-α (HRP)

3326-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT24A/44A/104
Detection mAbMT9L/14A/113, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IFN-α ELISA standard
Buffer/SolutionStandard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

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ELISA substrate: TMB for HRP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IFN-α in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IFN-α (HRP)
ApplicationELISA
AnalyteIFN-α pan
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of mouse IFN-α (pan specific). Subtypes 1, 2, 4, 5, 6T, 7/10 (129/Sv), 8/6, 9, 11, 12, 13, 14, 15 (A), and B are detected.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range8-1000 pg/ml
Representative standard curve for mouse IFN-α pan ELISA Flex kit using TMB substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published November 28, 2022

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Tutorial, Published July 31, 2023

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IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA