ELISA Flex: Human IFN‑α (ALP)

Standard curve for human IFN-α ELISA Flex kit.

ELISA Flex: Human IFN‑α (ALP)

3425-1A-6

Publications: 138

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT1/3/5
Detection mAb	MT2/4/6, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Standard	Recombinant human IFN-α ELISA standard
Buffer/Solution	Standard reconstitution buffer

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ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human IFN-α in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IFN-α (ALP)
Application	ELISA
Analyte	IFN-α pan
Reactivity	Human, Non-human primates
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of human IFN-α. Subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16, and 17 are detected.
Cross-reactivity	The mAbs cross-react with IFN-α from non-human primates (NHP). For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

4-400 pg/ml

Representative standard curve for human IFN-α ELISA Flex kit using pNPP substrate.

Datasheet/Protocol SDS

Tutorial

2025-04-08

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial

2023-09-20

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

2022-11-28

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells.

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative names

Interferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA

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ELISA Pro: Human IFN-α