ELISA Flex: Human Granzyme B (ALP)

Standard curve for human Granzyme B ELISA Flex kit.

ELISA Flex: Human Granzyme B (ALP)

3486-1A-6

Publications: 78

Documents

Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT34B6
Detection mAb	MT28, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Standard	Recombinant human granzyme B ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Frequently bought together

1 / 2

ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human granzyme B in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human Granzyme B (ALP)
Application	ELISA
Analyte	Granzyme B
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

10-1000 pg/ml

Representative standard curve for human Granzyme B ELISA Flex kit using pNPP substrate.

Datasheet/Protocol SDS

Tutorial

2025-04-08

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial

2023-09-20

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

2022-11-28

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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Granzyme B

Analyte description	Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
Alternative names	Granzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3002