ELISA Flex: Human CD25 (ALP)

ELISA Flex: Human CD25 (ALP)

3447-1A-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMTAW11A
Detection mAbMTAW9, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human CD25 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human CD25 (IL-2Rα) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human CD25 (ALP)
ApplicationELISA
AnalyteCD25 (IL-2Rα)
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human CD25 (IL-2 receptor α).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range10-3160 pg/ml

Representative standard curve for human CD25 ELISA Flex kit using pNPP substrate.

Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Featured product, Published March 3, 2023

Human CD25 ELISA launched
We have added monoclonal antibodies and ELISA Flex kits to detect human CD25 to our webshop.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.
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CD25 (IL-2Rα)

Analyte description

CD25 is expressed by activated T cells, B cells, and monocytes. Tregs, responsible for regulating immune tolerance, express the highest amounts of CD25. CD25 is the IL-2 receptor α chain, which functions as one subunit of the high-affinity variant of the IL-2 receptor. When cleaved off by proteases, the soluble receptor (sIL-2Rα) is released extracellularly. Soluble CD25 has a low affinity for IL-2 and can function either as a decoy receptor reducing the activity of IL-2 or by enabling trans presentation to cells expressing the dimeric, intermediate-affinity IL-2R. Increased levels of soluble CD25 indicate ongoing immune responses, and therefore CD25 has been investigated as a biomarker for disease. For example, elevated sCD25 levels have been associated with autoimmune disorders, severe COVID-19, and some types of cancer. 

Alternative namesCD25, cluster of differentiation 25, Interleukin-2 receptor subunit alpha, IL2RA, Tac, p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Cell typeT cell
Gene ID3559