ELISA Flex: Human CD25 (ALP)

Standard curve for human CD25 ELISA Flex kit.

ELISA Flex: Human CD25 (ALP)

3447-1A-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MTAW11A
Detection mAb	MTAW9, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Standard	Recombinant human CD25 ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Frequently bought together

1 / 2

ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human CD25 (IL-2Rα) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human CD25 (ALP)
Application	ELISA
Analyte	CD25 (IL-2Rα)
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human CD25 (IL-2 receptor α).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

10-3160 pg/ml

Representative standard curve for human CD25 ELISA Flex kit using pNPP substrate.

Datasheet/Protocol SDS

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CD25 (IL-2Rα)

Analyte description	CD25 is expressed by activated T cells, B cells, and monocytes. Tregs, responsible for regulating immune tolerance, express the highest amounts of CD25. CD25 is the IL-2 receptor α chain, which functions as one subunit of the high-affinity variant of the IL-2 receptor. When cleaved off by proteases, the soluble receptor (sIL-2Rα) is released extracellularly. Soluble CD25 has a low affinity for IL-2 and can function either as a decoy receptor reducing the activity of IL-2 or by enabling trans presentation to cells expressing the dimeric, intermediate-affinity IL-2R. Increased levels of soluble CD25 indicate ongoing immune responses, and therefore CD25 has been investigated as a biomarker for disease. For example, elevated sCD25 levels have been associated with autoimmune disorders, severe COVID-19, and some types of cancer.
Alternative names	CD25, cluster of differentiation 25, Interleukin-2 receptor subunit alpha, IL2RA, Tac, p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Cell type	T cell
Gene ID	3559