Anti-SARS-CoV-2 (N) mAb (MTCC25), unconjugated

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.
Anti-SARS-CoV-2 (N) mAb MTCC25 detection of SARS-CoV-2 nucleocapsid protein in indirect ELISA. And ELISA plate was coated with 0.1 μg/ml recombinant SARS-CoV-2 nucleocapsid protein. Anti-SARS-CoV-2 (N) mAb MTCC25 (biotinylated) was added at 0.000064-1 µg/ml and the plate was developed was detected using Streptavidin-HRP and TMB substrate.
Anti-SARS-CoV-2 nucleocapsid mAb MTCC25 used in Western Blot analysis.
Flow cytometry analysis of HEK293 cells transfected with genes for SARS-CoV-2 nucleocapsid (ancestral sequence) and stained with mAb MTCC25 (solid line). Dashed line shows staining of non-transfected cells.
Flow cytometry analysis of HEK293 cells transfected with genes for SARS-CoV-2 nucleocapsid (omicron BA.1) and stained with mAb MTCC25 (solid line). Dashed line shows staining of non-transfected cells.

Anti-SARS-CoV-2 (N) mAb (MTCC25), unconjugated

4401-5-100

$370

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Product specifications

Intended use

This monoclonal antibody enables specific detection of the SARS-CoV-2 nucleocapsid protein in FociSpot immunostaining of virus infected cells. The antibody can also be used in Western blot, ELISA, and flow cytometry.

Serum/Plasma samples

Applications
FociSpot immunostaining of SARS-CoV-2 nucleocapsid in focus forming assays. Antibody functionality in FociSpot was confirmed using SARS-CoV-2 infected Vero cells.

Functionality in Western blot and ELISA was confirmed using recombinant protein, and functionality in flow cytometry was confirmed using transfected HEK cells.

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Product details

ProductAnti-SARS-CoV-2 (N) mAb (MTCC25), unconjugated
ApplicationFociSpot, Western blot, ELISA, Flow cytometry
AnalyteSARS-CoV-2 (N)
AntibodyMTCC25
ConjugateUnconjugated
ClonalityMonoclonal
Immunogen

Recombinant SARS-CoV-2 nucleocapsid protein

HostMouse
IsotypeIgG1
ReactivityNaN
Specificity

Native and recombinant SARS-CoV-2 nucleocapsid protein

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MTCC25, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.
Anti-SARS-CoV-2 (N) mAb MTCC25 detection of SARS-CoV-2 nucleocapsid protein in indirect ELISA. And ELISA plate was coated with 0.1 μg/ml recombinant SARS-CoV-2 nucleocapsid protein. Anti-SARS-CoV-2 (N) mAb MTCC25 (biotinylated) was added at 0.000064-1 µg/ml and the plate was developed was detected using Streptavidin-HRP and TMB substrate.
Anti-SARS-CoV-2 nucleocapsid mAb MTCC25 used in Western Blot analysis.
Flow cytometry analysis of HEK293 cells transfected with genes for SARS-CoV-2 nucleocapsid (ancestral sequence) and stained with mAb MTCC25 (solid line). Dashed line shows staining of non-transfected cells.
Flow cytometry analysis of HEK293 cells transfected with genes for SARS-CoV-2 nucleocapsid (omicron BA.1) and stained with mAb MTCC25 (solid line). Dashed line shows staining of non-transfected cells.
Datasheet/ProtocolSDS

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SARS-CoV-2 (N)

Analyte description

The nucleocapsid (N) protein is a structural protein of the SARS-CoV-2 virus. It is responsible for packaging the positive-sense RNA genome by forming ribonucleoprotein (RNP) structures with the viral RNA. The N protein is mainly expressed in the host cells' cytoplasm and interacts with the M protein during assembly. The nucleocapsid protein is highly immunogenic and antibodies against it are found in people that recovered from COVID-19. The N protein has been suggested to suppress type I interferon responses. 

Alternative namesNucleocapsid protein, N protein (of SARS-CoV-2)