Anti-SARS-CoV-2 (N) mAb (MTCC25), biotin

Anti-SARS-CoV-2 (N) mAb (MTCC25), biotin

4401-6-100

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Content

Monoclonal antibody MTCC25, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Product specifications

Intended use

This monoclonal antibody enables specific detection of the SARS-CoV-2 nucleocapsid protein in FociSpot immunostaining of virus infected cells. The antibody can also be used in Western blot and ELISA.

Serum/Plasma samples

Applications
FociSpot immunostaining of SARS-CoV-2 nucleocapsid in focus forming assays. Antibody functionality in FociSpot was confirmed using SARS-CoV-2 infected Vero cells.

Functionality in Western blot and ELISA was confirmed using recombinant protein.

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Product details

ProductAnti-SARS-CoV-2 (N) mAb (MTCC25), biotin
ApplicationFociSpot, Western blot, ELISA
AnalyteSARS-CoV-2 (N)
AntibodyMTCC25
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant SARS-CoV-2 nucleocapsid protein

HostMouse
IsotypeIgG1
ReactivityNaN
Specificity

Native and recombinant SARS-CoV-2 nucleocapsid protein

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MTCC25, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of SARS-CoV-2 nucleocapsid in infected Vero cells. Vero E6 cells were infected with the SARS-CoV-2 virus (titrated). After fixation and permeabilization, foci were detected using anti-SARS-CoV-2 (N) mAb MTCC25, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/Protocol
SDS
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SARS-CoV-2 (N)

Analyte description

The nucleocapsid (N) protein is a structural protein of the SARS-CoV-2 virus. It is responsible for packaging the positive-sense RNA genome by forming ribonucleoprotein (RNP) structures with the viral RNA. The N protein is mainly expressed in the host cells' cytoplasm and interacts with the M protein during assembly. The nucleocapsid protein is highly immunogenic and antibodies against it are found in people that recovered from COVID-19. The N protein has been suggested to suppress type I interferon responses. 

Alternative namesNucleocapsid protein, N protein (of SARS-CoV-2)