Anti-porcine IL-2 mAb (MT265), biotin

3140-6-250

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Anti-porcine IL-2 mAbs (MT264/267), unconjugated

Intended use

This monoclonal antibody enables specific detection of porcine IL-2 in immunoassays such as ELISpot, FluoroSpot, and ELISA. It also enables specific detection of ferret IL-2 in ELISA and ELISpot.

Serum/Plasma samples

Recommendation

MT265 is recommended as detection mAb for porcine IL-2 in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs MT264/267 (product code 3140-3). For ferret IL-2, it is recommended in ELISA in combination with capture mAb MT264 (product code 3104-1) and in ELISpot in combination with capture mAbs MT264/271 (product code 3104-3).

Product details

Product	Anti-porcine IL-2 mAb (MT265), biotin
Application	ELISpot, FluoroSpot, ELISA
Analyte	IL-2
Antibody	MT265
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant porcine IL-2
Host	Mouse
Isotype	IgG2b
Reactivity	Ferret, Pig
Specificity	Native and recombinant porcine (pig) IL-2
Cross-reactivity	Cross-reacts with IL-2 from ferret. For more information, please check the Veterinary cross-reactivity guide.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT265, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

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IL-2

Analyte description	Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
Alternative names	Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, POIL2, TCGF
Cell type	T cell, Th1, Th2, Th17, Tfh
Gene ID	396868, 101674110