Anti-porcine IFN-γ mAb (pIFNγ-I), unconjugated

3130-3-250

Publications: 75

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Anti-porcine IFN-γ mAb (P2C11), biotin

Intended use

This monoclonal antibody enables specific detection of porcine IFN-γ in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

pIFNγ-I is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb P2C11 (product code 3130-6).

Product details

Product	Anti-porcine IFN-γ mAb (pIFNγ-I), unconjugated
Application	ELISpot, FluoroSpot, ELISA
Analyte	IFN-γ
Antibody	pIFNγ-I
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Recombinant porcine IFN-γ
Host	Mouse
Isotype	IgG1
Reactivity	Pig
Specificity	Native porcine (pig) IFN-γ. Recombinant porcine IFN-γ produced in E.coli and human cells. No reactivity with recombinant porcine IFN-γ produced in yeast.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody pIFNγ-I, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	396991

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ELISA Flex: Porcine IFN-γ (HRP)