Anti-porcine IFN-γ mAb (P2C11), FITC

Interferon-gamma (IFN-γ) flow cytometry

Anti-porcine IFN-γ mAb (P2C11), FITC

Intended use

This monoclonal antibody enables specific detection of porcine IFN-γ-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl.

Product details

ProductAnti-porcine IFN-γ mAb (P2C11), FITC
ApplicationFlow cytometry
AnalyteIFN-γ
AntibodyP2C11
ConjugateFITC
ClonalityMonoclonal
ImmunogenRecombinant porcine IFN-γ
HostMouse
IsotypeIgG2a
ReactivityPig
Specificity

Native and recombinant porcine (pig) IFN-γ

Supplied in

PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody P2C11, conjugated to FITC fluorophore. Ready to use.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8 °C.

Shelf lifeAt least 12 months from date of receipt.

Interferon-gamma (IFN-γ) flow cytometry
Detection of IFN-γ by flow cytometry in viable porcine PBMC. Stimulated, fixed and permeabilized cells were stained with anti-porcine CD3-PE and anti-porcine IFN-γ mAb P2C11-FITC (right panel). Matched isotype control antibody (left panel). Flow cytometry was performed on a BD FACSVerse system.

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