Anti-influenza B (NP) mAb (4D5), biotin

Anti-influenza B (NP) mAb (4D5), biotin

4406-6-100

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Content

Monoclonal antibody 4D5, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of the influenza B nucleoprotein (NP) in FociSpot immunostaining of virus infected cells. The antibody can also be used in ELISA.

Serum/Plasma samples

Applications
FociSpot immunostaining of influenza B nucleoprotein in focus forming assays. Antibody functionality in FociSpot was confirmed using influenza B infected MDCK cells.

Functionality in ELISA was confirmed using recombinant protein.

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Product details

ProductAnti-influenza B (NP) mAb (4D5), biotin
ApplicationFociSpot, ELISA
AnalyteInfluenza B (NP)
Antibody4D5
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant influenza B nucleoprotein

HostMouse
IsotypeIgG1
Specificity

Native and recombinant influenza B nucleoprotein

Purification

Purified by protein A affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody 4D5, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

FociSpot immunostaining of influenza B nucleoprotein in infected MDCK cells. MDCK cells were infected with influenza B virus (titrated, virus dilutions16,000x, 32,000x, and 64,000x are shown). After fixation and permeabilization, foci were detected using anti-influenza B (NP) mAb 4D5, followed by Streptavidin-ALP and BCIP/NBT-plus substrate. Foci were counted using the Apex software in Mabtech IRIS 2.

Datasheet/Protocol
SDS

Featured product, Published June 21, 2023

Influenza B FociSpot kit is here
The nucleoprotein of Influenza B can be detected with our new monoclonal antibody, which is included in our corresponding FociSpot kit.
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Influenza B (NP)

Analyte description

The Nucleoprotein (NP) is a structural protein and encapsidates the negative strand RNA of influenza B into ribonucleoproteins (RNP). NP plays a role in replication, transcription, RNA packaging, and nuclear trafficking. The NP does not only interact with the RNA, but also the viral polymerase, the M protein, and several host factors. 

Alternative namesNucleoprotein, NP (of influenza B)