Anti-human TNF-α mAb (MT15B15), PF647P

Anti-human TNF-α mAb (MT15B15), PF647P

3512-72-100T

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Monoclonal antibody MT15B15, conjugated to PF647P fluorophore. Ready to use.

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Isotype control mAb (MT128) for mouse IgG1, PF647P
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3612-72-100T

Product specifications

Intended use

This monoclonal antibody enables specific detection of human TNF-α-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.

Product details

ProductAnti-human TNF-α mAb (MT15B15), PF647P
ApplicationFlow cytometry
AnalyteTNF-α
AntibodyMT15B15
ConjugatePF647P
ClonalityMonoclonal
ImmunogenRecombinant human TNF-α
HostMouse
IsotypeIgG1
ReactivityHuman
SpecificityNative and recombinant human TNF-α
Supplied inPBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT15B15, conjugated to PF647P fluorophore. Ready to use.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Detection of TNF-α by flow cytometry in viable human lymphocytes. Stimulated, fixed and permeabilized cells were stained with anti-human TNF-α mAb MT15B15-PF647P in combination with anti-CD3-PE (left panel). Matched isotype control antibody (right panel). Flow cytometry was performed on a BD FACSVerse system.
Datasheet/ProtocolSDS
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