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Anti-human TNF-α mAb (detection), biotin

Standard curve for human TNF‑α in bead-based multiplex.

Anti-human TNF-α mAb (detection), biotin

5335-6-100

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Anti-human TNF-α mAb (capture), unconjugated

Intended use

This monoclonal antibody enables specific detection of human TNF-α in bead-based multiplex assays. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Anti-human TNF-α mAb (detection) is recommended in combination with Anti-human TNF-α mAb (capture) (product code 5335-3) in bead-based multiplex.

Product details

ProductAnti-human TNF-α mAb (detection), biotin
ApplicationBead-based multiplex
AnalyteTNF-α
AntibodyAnti-human TNF-α mAb (detection)
ConjugateBiotin
ClonalityMonoclonal
ReactivityHuman
Specificity

Native and recombinant human TNF-α

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Anti-human TNF-α mAb (detection), biotin. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C.

Shelf lifeAt least 12 months from date of receipt.

Standard curve for human TNF‑α in bead-based multiplex.

Representative standard curve for human TNF‑α in bead-based multiplex.

Datasheet/ProtocolSDS

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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124