Anti-human IL-1α mAb (MT513), biotin

3414-6-250

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Anti-human IL-1α mAbs (MT641/642), unconjugated

Intended use

This monoclonal antibody enables specific detection of human IL-1α in immunoassays such as ELISpot, FluoroSpot, ELISA, and Western blot.

Serum/Plasma samples

Recommendation

MT513 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAbs MT641/642 (product code 3414-3). The antibody is also suitable for Western blot.

Product details

Product	Anti-human IL-1α mAb (MT513), biotin
Application	ELISpot, FluoroSpot, ELISA, Western blot
Analyte	IL-1α
Antibody	MT513
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant human IL-1α
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Native and recombinant human IL-1α. The antibody binds to the pro-form and the active form of IL-1α.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT513, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

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IL-1α

Analyte description	Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation.
Alternative names	Interleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1
Cell type	Monocyte/MΦ, mDC
Gene ID	3552

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ELISA Flex: Human IL-1α (HRP)