Anti-human IgA1 mAb (MT50), biotin

3861-6-250

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Anti-human IgA1 mAb (MT50), unconjugated

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Anti-human IgA1 mAb (MT50), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgA1 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT50 is recommended as detection mAb in ELISpot in combination with capture mAb MT57 (product code 3860-3). The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-human IgA1 mAb (MT50), biotin
Application	ELISpot, ELISA
Analyte	IgA1
Antibody	MT50
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native human IgA
Host	Rat
Isotype	IgG1
Reactivity	Human
Specificity	Human IgA1 derived from serum and colostrum
Cross-reactivity	No cross-reactivity (less than 0.1%) with human IgA2, IgG1, IgG2, IgG3, IgG4, IgE, or IgM
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT50, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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Datasheet/Protocol SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

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Analyte information

IgA1

Analyte description	IgA1 (Immunoglobulin A1) is one of the two existing IgA isotypes. The proportions of IgA1 and IgA2 secreting B cells differs in various body tissues. Although IgA1 is the most common IgA subclass in serum, IgA represents only 10-15% of the total serum Ig molecules. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.
Alternative names	Immunoglobulin A1, IgA1
Cell type	B cell
Gene ID	3493