Anti-human IFN-α mAb (MT2/4/6), biotin

Anti-human IFN-α mAb (MT2/4/6), biotin

3425-6-250

Publications: 130
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Content

Monoclonal antibody MT2/4/6, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

These monoclonal antibodies enable specific detection of human IFN-α subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17 (pan-specific detection) in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT2/4/6 are recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MT1/3/5 (product code 3425-3).

Product details

ProductAnti-human IFN-α mAb (MT2/4/6), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIFN-α pan
AntibodyMT2/4/6
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human IFN-α
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Native and recombinant human IFN-α. MT2/4/6 in combination with antibodies MT1/3/5 binds subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17.

Cross-reactivity

Cross-reacts with native IFN-α from rhesus macaque and cynomolgus macaque. For information on reactivity with other non-human primates (NHP), please check the NHP cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT2/4/6, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

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IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA