Anti-human IFN-α mAb (MT1/3/5), unconjugated

Anti-human IFN-α mAb (MT1/3/5), unconjugated


Publications: 130
Validated for our assays.


Monoclonal antibody MT1/3/5, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 1

Product specifications

Intended use

These monoclonal antibodies enable specific detection of human IFN-α subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17 (pan-specific detection) in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples


MT1/3/5 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb MT2/4/6 (product code 3425-6).

Product details

ProductAnti-human IFN-α mAb (MT1/3/5), unconjugated
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIFN-α pan
ImmunogenRecombinant human IFN-α
ReactivityHuman, Non-human primates

Native and recombinant human IFN-α. MT1/3/5 in combination with antibodies MT2/4/6 binds subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17.


Cross-reacts with native IFN-α from rhesus macaque and cynomolgus macaque. For information on reactivity with other non-human primates (NHP), please check the NHP cross-reactivity guide.


Purified from in vitro cultures by protein G affinity chromatography.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).


Monoclonal antibody MT1/3/5, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage


Shipped at ambient temperature.


Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
Loading publications...

IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA