Anti-human IFN-α mAb (MT1/3/5), unconjugated

Anti-human IFN-α mAb (MT1/3/5), unconjugated

3425-3-250

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Content

Monoclonal antibody MT1/3/5, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IFN-α mAb (MT2/4/6), biotin
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  • Code: 3425-6-250
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Product specifications

Intended use

These monoclonal antibodies enable specific detection of human IFN-α subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17 (pan-specific detection) in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT1/3/5 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb MT2/4/6 (product code 3425-6).

Product details

ProductAnti-human IFN-α mAb (MT1/3/5), unconjugated
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIFN-α pan
AntibodyMT1/3/5
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant human IFN-α
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Native and recombinant human IFN-α. MT1/3/5 in combination with antibodies MT2/4/6 binds subtypes 1/13, 2, 4, 5, 6, 7, 8, 10, 14, 16 and 17.

Cross-reactivityCross-reacts with native IFN-α from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT1/3/5, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA
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