Anti-human IFN-γ mAb (1-D1K), unconjugated

Model of human IFN-γ protein showing the epitopes for monoclonal antibodies 1-D1K (blue) and 7-B6-1 (orange).

Anti-human IFN-γ mAb (1-D1K), unconjugated

3420-3-250

Publications: 2227

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Anti-human IFN-γ mAb (7-B6-1), biotin

Intended use

This monoclonal antibody enables specific detection of human IFN-γ in immunoassays such as ELISpot, FluoroSpot, ELISA, and immunocytochemistry.

Serum/Plasma samples

Recommendation

1-D1K is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb 7-B6-1 (product code 3420-6). The antibody is also suitable for immunocytochemistry.

Product details

Product	Anti-human IFN-γ mAb (1-D1K), unconjugated
Application	ELISpot, FluoroSpot, ELISA, Immunocytochemistry
Analyte	IFN-γ
Antibody	1-D1K
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Recombinant human IFN-γ
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Native and recombinant human IFN-γ
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	1 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody 1-D1K, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Model of human IFN-γ protein showing the epitopes for monoclonal antibodies 1-D1K (blue) and 7-B6-1 (orange).

Datasheet/Protocol SDS

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Discover the PepPool: CEFRAS Global family of peptide pools delivering superior T cell stimulation across diverse populations. With meticulously chosen epitopes from a number of common pathogens, it sets a new standard for ELISpot, FluoroSpot, and flow cytometry.

Highlighted research

ELISpot important in evaluating SARS-CoV-2 vaccine efficacy

Two studies that utilized Mabtech's ELISpot IFN-γ kits to evaluate spike protein-specific T cell responses pre- and post-vaccination.

Highlighted research

T cell immunity in COVID-19

Much emphasis has been put on antibody responses, but also T cells are important in the context of COVID-19. In this study, the authors characterized SARS-CoV-2-specific T cell responses by using an extensive FACS panel, peptide stimulations, tetramer staining, and Mabtech IFN-γ ELISpot.

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IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3458

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ELISpot Plus: Human IFN-γ (ALP)