Anti-human CD28 mAb (CD28-A), azide free

Anti-human CD28 mAb (CD28-A), azide free

3608-1-50

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Content

Monoclonal antibody CD28-A, unconjugated. Supplied at 100 µg/ml in sterile filtered PBS, free of preservatives.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This monoclonal antibody enables co-stimulation of human T cells.

Serum/Plasma samples

Recommendation

CD28-A can be used together with antigen to co-stimulate specific T cells. The recommended working concentration of CD28-A is 0.1 µg/ml.

Product details

ProductAnti-human CD28 mAb (CD28-A), azide free
ApplicationT cell stimulation
AnalyteCD28
AntibodyCD28-A
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenStimulated human leukocytes
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Human CD28

Cross-reactivity

Cross-reacts with CD28 from rhesus macaque and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration100 µg/ml
Supplied in

PBS, free of preservatives. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody CD28-A, unconjugated. Supplied at 100 µg/ml in sterile filtered PBS, free of preservatives.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

 Rhesus MacaqueCynomolgus MacaquePig-tailed MacaqueBaboon*Sooty MangabeyAfrican Green MonkeyNight Monkey*Common MarmosetSquirrel Monkey
ASSAYOld worldNew world
ApoA1      
ApoB       
ApoE (NHP)       
ApoE       
ApoH       
CCL2 (MCP-1)       
CCL22 (MDC) ( ) ( )( )( )( )
CD25       
GM-CSF( )( )   
Granzyme A        
Granzyme B (NHP)       
Granzyme B     
IFN-α2        
IFN-α pan   ( )   
IFN-γ (NHP)
IFN-γ( )
IgA (NHP)       
IgA       
IgG       
IgM       
INS     
IL-1α        
IL-1β        
IL-2 (NHP)
IL-3       
IL-4( )( )( )( )
IL-5( )( )( ) 
IL-6( )( ) ( ) 
IL-8 (NHP)        
IL-8 (ELISA)        
IL-8 (ELISpot)        
IL-10 (NHP)      
IL-10       
IL-12/-23 (p40)      
IL-12 (p70)        
IL-13 (NHP)( )( )( )  
IL-13        
IL-17A (NHP)( )( )   
IL-17A     
IL-17F        
IL-17A/F        
IL-21      
IL-22        
IL-23     
IL-27        
IL-31        
IP-10       
Perforin( )   
TGF-β1 (latent)       
TNF-α (NHP)( )  ( )( )( )( )
          
SINGLE mAbs         
Cell stimulation         
CD3, mAb CD3-1       
CD3, mAb CD3-2       
CD28, mAb CD28-A       
Flow cytometry          
IFN-γ mAb 1-D1K( )( )( )( )( )( )
IL-2 mAb MT8G10( )( )( )( ) ( )( )( )
IL-4 mAb IL4-3( )       
IL-17A mAb MT504        
Perforin mAb Pf-344     
TNF-α mAb MT15B15       
Ig reagents         
IgG1 mAb MTG1218       
IgG1 mAb MT1939       
IgG2 mAb H6200       
IgG2 mAb MTG211E       
IgG3 mAb MTG34       
IgG4 mAb MTG42       
Western blot         
IFN-γ mAb MT111W( )( )( )( )( )( )( )( )( )
TNF-α mAb MT15B15( )( )( )  ( )( )( )( )

Symbol keys:


Cross-reactivity

Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

  • good
  • poor
  • no

Indications of Cross-reactivity

Based on reports from others or by analysis using recombinant proteins.

  • ( ) good
  • ( ) poor
  • ( ) no

Monkey kits

Specifically developed monkey kits are marked with (NHP).

Blank boxes

Represent cross-reactivities not evaluated.

* Comprises several species. Cross-reactivity may have to be verified on a species basis.

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CD28

Analyte description

CD28 (cluster of differentiation 28) is a 44 kDa homodimeric transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD28 is mainly expressed on T cells, including thymocytes and may be found on plasmablasts. CD28 binds to CD80 (B7.1) and CD86 (B7.2), both being expressed by antigen-presenting cells, providing co-stimulatory signals to T cells via its intracellular domain.

Alternative names, Tp44
Cell typeT cell, Tc, Th1, Th2, Th17, Treg, Tfh
Gene ID940