Anti-human CD25 mAb (MTAW9), biotin

Anti-human CD25 mAb (MTAW9), biotin

3447-6-250

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Content

Monoclonal antibody MTAW9, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human CD25 mAb (MTAW11A), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of human CD25 (IL-2Rα) in ELISA.

Serum/Plasma samples

Recommendation

MTAW9 is recommended as detection mAb in ELISA in combination with capture mAb MTAW11A (product code 3447-3).

Product details

ProductAnti-human CD25 mAb (MTAW9), biotin
ApplicationELISA
AnalyteCD25 (IL-2Rα)
AntibodyMTAW9
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant soluble human CD25

HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Native and recombinant human CD25 (IL-2 receptor α)

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MTAW9, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Featured product, Published March 3, 2023

Human CD25 ELISA launched
We have added monoclonal antibodies and ELISA Flex kits to detect human CD25 to our webshop.
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CD25 (IL-2Rα)

Analyte description

CD25 is expressed by activated T cells, B cells, and monocytes. Tregs, responsible for regulating immune tolerance, express the highest amounts of CD25. CD25 is the IL-2 receptor α chain, which functions as one subunit of the high-affinity variant of the IL-2 receptor. When cleaved off by proteases, the soluble receptor (sIL-2Rα) is released extracellularly. Soluble CD25 has a low affinity for IL-2 and can function either as a decoy receptor reducing the activity of IL-2 or by enabling trans presentation to cells expressing the dimeric, intermediate-affinity IL-2R. Increased levels of soluble CD25 indicate ongoing immune responses, and therefore CD25 has been investigated as a biomarker for disease. For example, elevated sCD25 levels have been associated with autoimmune disorders, severe COVID-19, and some types of cancer. 

Alternative namesCD25, cluster of differentiation 25, Interleukin-2 receptor subunit alpha, IL2RA, Tac, p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Cell typeT cell
Gene ID3559