Anti-human apoM mAb (M5), unconjugated

Anti-human apoM mAb (M5), unconjugated

3716-3-250

Publications: 2
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Content

Monoclonal antibody M5 unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human apoM mAb (M7), biotin
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  • Code: 3716-6-250
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Product specifications

Intended use

This monoclonal antibody enables specific detection of human apoM in immunoassays such as ELISA, immunoprecipitation and Western blot.

Serum/Plasma samples

Recommendation

M5 is recommended as capture mAb in ELISA in combination with biotinylated detection mAb M7 (product code 3716-6). The antibody is also suitable for immunoprecipitation and Western blot.

Product details

ProductAnti-human apoM mAb (M5), unconjugated
ApplicationELISA, Western blot
AnalyteapoM
AntibodyM5
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant human apoM
HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Native and recombinant human apoM

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody M5 unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoM with mAb M5 in Western blot. 0.1 µl human plasma and 5ng purified apoM in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoM was detected using 2 µg/ml M5 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.
Datasheet/ProtocolSDS

apoM

Analyte description

Apolipoprotein M (apoM, also known as apolipoproteinM and apo M) is a lipocalin mainly produced by the liver. It binds sphingosine-1-phosphate (S1P), and HDL containing apoM is the major transporter of S1P. ApoM–S1P is involved in several signaling pathways implicated in the atheroprotective functions of HDL. ApoM has a role in lipoprotein metabolism, atherosclerosis, diabetes, and renal diseases. Normal concentration of apoM in human plasma is approximately 6-60 mg/L.

Alternative names, G3a, HSPC336, NG20, apo-M
Cell typeB cell, Monocyte/MΦ, mDC
Gene ID55937
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Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs