Anti-human apoH mAb (H464), biotin

Anti-human apoH mAb (H464), biotin

3711-6-250

Publications: 4
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Content

Monoclonal antibody H464, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Apo ELISA buffer
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  • Code: 3652-M2
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Product specifications

Intended use

This monoclonal antibody enables specific detection of apoH in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

H464 is recommended as detection mAb in ELISA in combination with capture mAb H219 (product code 3711-3). The antibody is also suitable for Western blot.

Product details

ProductAnti-human apoH mAb (H464), biotin
ApplicationELISA, Western blot
AnalyteapoH
AntibodyH464
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative human apoH
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Human apolipoprotein H (apoH)

Cross-reactivityCross-reacts with apoH from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody H464, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoH with mAb H464 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoH was detected using 1 µg/ml H464 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.
Datasheet/ProtocolSDS

apoH

Analyte descriptionApolipoprotein H (apoH, also known as apolipoproteinH, apo H, beta 2-glycoprotein 1 and β2-glycoprotein I) is associated to VLDL, HDL and chylomicrons in plasma and binds to anionic lipids e.g. phospholipids. ApoH is the dominant target of autoantibodies in anti-phosphlipid syndrome (APS). In the blood coagulation pathway, apoH is involved both as an anti-coagulant and as a procoagulant. Normal concentration of apoH in human plasma is approximately 0.05-0.6 g/L.
Alternative names, B2G1, B2GP1, BG
Gene ID350
Loading publications...

Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs

Cross-reactivity guide, Published November 21, 2022

Non-human primate cross-reactivity
Mabtech offers a broad range of ELISpot kits in different formats for the detection of cytokines and other immunological effector molecules in samples from non-human primates (NHP).