Anti-human apoH mAb (H219), unconjugated

Detection of human apoH with mAb H219 in Western blot.

Anti-human apoH mAb (H219), unconjugated

3711-3-250

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Anti-human apoH mAb (H464), biotin

Intended use

This monoclonal antibody enables specific detection of apoH in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

H219 is recommended as capture mAb in ELISA in combination with detection mAb H464 (product code 3711-6). The antibody is also suitable for Western blot.

Product details

Product	Anti-human apoH mAb (H219), unconjugated
Application	ELISA, Western blot
Analyte	apoH
Antibody	H219
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Native human apoH
Host	Mouse
Isotype	IgG1
Reactivity	Human, Non-human primates
Specificity	Human apolipoprotein H (apoH)
Cross-reactivity	Cross-reacts with apoH from rhesus macaque and cynomolgus macaque. For information on reactivity with other non-human primates (NHP), please check the NHP cross-reactivity guide.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody H219, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Detection of human apoH with mAb H219 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoH was detected using 1 µg/ml H219 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.

Datasheet/Protocol SDS

Tutorial

Apolipoprotein dilution

Recommendations for Mabtech's apolipoprotein ELISA kits

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apoH

Analyte description	Apolipoprotein H (apoH) is associated to VLDL, HDL, and chylomicrons in plasma and binds to anionic lipids e.g., phospholipids. ApoH is the dominant target of autoantibodies in anti-phospholipid syndrome (APS). In the blood coagulation pathway, apoH is involved both as an anti-coagulant and as a procoagulant. The normal concentration of apoH in human plasma is approximately 0.05-0.6 g/L.
Alternative names	Apolipoprotein H, apoH, apolipoproteinH, apo H, beta 2-glycoprotein 1, β2-glycoprotein I, B2G1, B2GP1, BG
Gene ID	350

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ELISA Pro: Human apoH