Anti-cat IFN-γ mAb (MT114), biotin

Anti-cat IFN-γ mAb (MT114), biotin

3122-6-250

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Content

Monoclonal antibody MT114, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

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Product specifications

Intended use

This monoclonal antibody enables specific detection of cat IFN-γ in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT114 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb MT131 (product code 3122-3).

Product details

ProductAnti-cat IFN-γ mAb (MT114), biotin
ApplicationELISpot, ELISA
AnalyteIFN-γ
AntibodyMT114
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant cat IFN-γ
HostMouse
IsotypeIgG1
ReactivityCat, Lion, Cheetah, Leopard
Specificity

Native and recombinant cat IFN-γ

Cross-reactivity

Cross-reacts with IFN-γ from lion, cheetah, and leopard. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT114, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID493965
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