Anti-bovine IL-8 (CXCL8) mAb (26E5), biotin

3114-6-250

Publications: 26

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Intended use

This monoclonal antibody enables specific detection of bovine IL-8 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

26E5 is recommended as detection mAb in ELISpot and FluoroSpot in combination with capture mAbs MT8H6/8F19 (product code 3114-1), and in ELISA in combination with capture mAb MT8H6 (product code 3114-3).

Product details

Product	Anti-bovine IL-8 (CXCL8) mAb (26E5), biotin
Application	ELISpot, FluoroSpot, ELISA
Analyte	IL-8 (CXCL8)
Antibody	26E5
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant human IL-8
Host	Mouse
Isotype	IgG2b
Reactivity	Cow, Dog
Specificity	Native and recombinant bovine (cow) IL-8. Native and recombinant human IL-8.
Cross-reactivity	Cross-reacts with IL-8 from dog. For more information, please check the Veterinary cross-reactivity guide.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody 26E5, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

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IL-8 (CXCL8)

Analyte description	Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.
Alternative names	Interleukin-8, IL-8, IL8, CXCL8
Cell type	T cell, Treg, Tfh, Monocyte/MΦ, mDC
Gene ID	280828, 403850

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Anti-bovine IL-8 (CXCL8) mAbs (MT8H6/8F19), unconjugated