Anti-bovine IL-8 (CXCL8) mAb (26E5), biotin

Anti-bovine IL-8 (CXCL8) mAb (26E5), biotin

3114-6-250

Publications: 22
Documents
Questions?
Validated for our assays.

Content

Monoclonal antibody 26E5, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IL-8 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

26E5 is recommended as detection mAb in ELISpot and FluoroSpot in combination with capture mAbs MT8H6/8F19 (product code 3114-1), and in ELISA in combination with capture mAb MT8H6 (product code 3114-3).

Product details

ProductAnti-bovine IL-8 (CXCL8) mAb (26E5), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-8 (CXCL8)
Antibody26E5
ConjugateBiotin
ClonalityMonoclonal
Immunogen

Recombinant human IL-8

HostMouse
IsotypeIgG2b
ReactivityCow, Dog
Specificity

Native and recombinant bovine (cow) IL-8. Native and recombinant human IL-8.

Cross-reactivity

Cross-reacts with IL-8 from dog. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody 26E5, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
Loading publications...

IL-8 (CXCL8)

Analyte description

Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.

Alternative namesInterleukin-8, IL-8, IL8, CXCL8
Cell typeT cell, Treg, Tfh, Monocyte/MΦ, mDC
Gene ID280828, 403850
1 / 2