Anti-bovine IL-2 mAb (MT3B3), PF647P

3111-72-100T

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Content

Monoclonal antibody MT3B3, conjugated to PF647P fluorophore. Ready to use.

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Anti-bovine IL-2 mAb (MT3B3), PF488P

Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IL-2-producing cells in flow cytometry.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.

Product details

Product	Anti-bovine IL-2 mAb (MT3B3), PF647P
Application	Flow cytometry
Analyte	IL-2
Antibody	MT3B3
Conjugate	PF647P
Clonality	Monoclonal
Immunogen	Recombinant bovine IL-2
Host	Mouse
Isotype	IgG1
Reactivity	Cow, Sheep
Specificity	Native and recombinant bovine (cow) IL-2
Supplied in	PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT3B3, conjugated to PF647P fluorophore. Ready to use.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Detection of IL-2 by flow cytometry in viable bovine lymphocytes. Stimulated, fixed and permeabilized cells were stained with anti-bovine mAb MT3B3-PF647P (solid line). Matched isotype control antibody (dashed line). Flow cytometry was performed on a BD FACSVerse system.

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Analyte information

IL-2

Analyte description	Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
Alternative names	Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell type	T cell, Th1, Th2, Th17, Tfh
Gene ID	280822