Anti-bovine IL-2 mAb (MT11A31), unconjugated

Anti-bovine IL-2 mAb (MT11A31), unconjugated

3111-3-250

Publications: 2
Documents
Questions?
Validated for our assays.

Content

Monoclonal antibody MT11A31, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 1

Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IL-2 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT11A31 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb MT3B3 (product code 3111-6).

Product details

ProductAnti-bovine IL-2 mAb (MT11A31), unconjugated
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-2
AntibodyMT11A31
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant bovine IL-2
HostMouse
IsotypeIgG1
ReactivityCow
SpecificityNative and recombinant bovine (cow) IL-2
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration1 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT11A31, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Loading publications...

IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID280822
1 / 2