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Anti-bovine IL-17A mAb (MT49A7), unconjugated

Standard curve for bovine, sheep and goat IL-17A ELISA Flex kit.
Bovine PBMC were incubated overnight in the presence (40,000 cells/well) or absence (200,000 cells/well) of PHA and the number of cells secreting IL-17A was analyzed by ELISpot.
Sheep PBMC (100,000 cells/well) were incubated overnight in the presence or absence of ConA and the number of cells secreting IL-17A was analyzed by ELISpot.
Goat PBMC (100,000 cells/well) were incubated overnight in the absence of PHA and the number of cells secreting IL-17A was analyzed by ELISpot.

Anti-bovine IL-17A mAb (MT49A7), unconjugated

3109-3-250

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Anti-bovine IL-17A mAb (MT51B8), biotin

Intended use

This monoclonal antibody enables specific detection of bovine, sheep, goat, and porcine IL-17A in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT49A7 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb MT51B8 (product code 3109-6) for detection of IL-17A from cow, sheep, and goat. For detection of IL-17A from pig, MT49A7 should be used in combination with detection mAb MTP853 (product code 3020-6).

Product details

ProductAnti-bovine IL-17A mAb (MT49A7), unconjugated
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-17A
AntibodyMT49A7
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant bovine IL-17A
HostMouse
IsotypeIgG1
ReactivityCow, Goat, Sheep, Pig
Specificity

Native and recombinant bovine (cow) IL-17A

Cross-reactivity

Cross-reacts with IL-17A from sheep, goat, and pig. For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT49A7, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Standard curve for bovine, sheep and goat IL-17A ELISA Flex kit.
Representative standard curve for bovine, sheep and goat IL-17A ELISA Flex kit using pNPP substrate.
Standard curve for bovine, sheep and goat IL-17A ELISA Flex kit.
Bovine PBMC were incubated overnight in the presence (40,000 cells/well) or absence (200,000 cells/well) of PHA and the number of cells secreting IL-17A was analyzed by ELISpot.
Sheep PBMC (100,000 cells/well) were incubated overnight in the presence or absence of ConA and the number of cells secreting IL-17A was analyzed by ELISpot.
Goat PBMC (100,000 cells/well) were incubated overnight in the absence of PHA and the number of cells secreting IL-17A was analyzed by ELISpot.

Datasheet/ProtocolSDS

Featured product

First porcine IL-17A spot assays

Analyze Th17 cells in pigs with ELISpot and FluoroSpot.

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IL-17A

Analyte description

Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.

Alternative namesInterleukin 17A, IL-17A, IL17A, IL-17, IL17
Cell typeTh17
Gene ID282863