ELISA Flex: Canine IFN‑γ (HRP)

Standard curve for canine IFN-γ ELISA Flex kit.

ELISA Flex: Canine IFN‑γ (HRP)

3113-1H-6

Publications: 5

Documents

Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT13
Detection mAb	MT166, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant canine IFN-γ ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This ELISA Flex kit is intended for the quantification of canine IFN-γ in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Canine IFN-γ (HRP)
Application	ELISA
Analyte	IFN-γ
Reactivity	Dog
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of canine (dog) IFN-γ.
Cross-reactivity	The mAbs react with horse IFN-γ and cross-react with IFN-γ from dog. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

8-800 pg/ml

Representative standard curve for canine IFN-γ ELISA Flex kit using TMB substrate.

Datasheet/Protocol SDS

Tutorial

2025-04-08

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial

2023-09-20

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

2022-11-28

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Highlighted research

2019-05-28

Canine ELISA in drug discovery

Controlling fusion protein stability and pharmacokinetic with Mabtech ELISA Flex: Canine IFN-γ.

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IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFN-G
Cell type	T cell, Tc, Th1, NK cell
Gene ID	403801